Detection method details
Name: |
QT-EVE-BN-010 |
Description: |
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape event MON88302 (EU-RL GMFF GMOMETHODS database as of January 27, 2014) |
Comment: |
Target is the 5' integration border region (IBR) between the insert of oilseed rape event MON88302 and the oilseed rape host genome |
Validation: |
ring trial validation |
Standardisation: |
EU reference method |
Related Methods: |
QT-TAX-BN-002 |
Type: |
event-specific |
Target GMO name: |
MON88302 |
-
Oligonucleotides:
Forward Primer
Name:
88302QFSequence:
TCCTTGAACCTTATTTTATAGTGCACASize:
27
Reverse Primer
Name:
88302QRSequence:
TCAGATTGTCGTTTCCCGCCTTCASize:
24
Probe
Name:
88302QPSequence:
TAGTCATCATGTTGTACCACTTCAAACACTSize:
30
-
Amplicon:
Sequence:
tccttgaaccttattttatagtgcacannnnnnnntagtcatcatgttgtaccacttcaaacactnnnnnnnnnnnntgaaggcgggaaacgacaatctgaSize:
101
Documents
Citation | Type | Local copy |
---|---|---|
EU-RL GMFF GMOMETHODS database; QT-EVE-BN-010 | EU reference method protocol |