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Detection Image Detection method details

Name:

 QL-CON-T51-SF-2/OsNOS-R2

Description:

 Qualitative real-time PCR (TaqMan) method for detection of the junction between the Bt toxin cryIAc gene and the terminator region of the nopaline synthase gene (T-nos) (Sampling and analysis guidelines for the detection of genetic modifications in rice, BVL; Akiyama et al., 2007)

Comment:

 Target is the junction between the Bt toxin cryIAc gene from Bacillus thuringiensis and the terminator region of the nopaline synthase gene (T-nos) from Agrobacterium tumefaciens.

To obtain better consensus of primer T51-SF-2 with known DNA sequences (see GenBank Nr. HQ161057 = Kefeng6), this primer sequence is optimised.

Validation:

 unknown

Standardisation:

 none

Related Methods:

Type:

 construct-specific

Target GMO names:
Kefeng6

  • Oligonucleotides:

  • Forward Primer

  • Name:

    T51-SF-2

  • Sequence:

    GCAGGAGTGATTATCGACAGATTC

  • Size:

    24

  • Reverse Primer

  • Name:

    OsNOS-R2

  • Sequence:

    AAGACCGGCAACAGGATTCA

  • Size:

    20

  • Probe

  • Name:

    NGMr-Taq

  • Sequence:

    AATGAGAATTCGGTACCCCGACCTGCA

  • Size:

    27

  • Amplicon:

  • Sequence:

    gcaggagtgattatcgacagattcgagttcattccagttactgcaacactcgaggctgaatgagaattcggtaccccgacctgcagatcgttcaaacatttggcaataaagtttcttaagattgaatcctgttgccggtctt

  • Size:

    142

Documents

Citation Type Local copy
Sampling and analysis guidelines for the detection of genetic modifications in riceLink to the document url. PCR Protocol Download PDF pdf
Akiyama H., Sasaki N., Sakata K., Ohmori K., Toyota A., Kikuchi Y., Watanabe T., Furui S., Kitta K., Maitani T. (2007) Indicated detection of two unapproved transgenic rice lines contaminating vermicelli products. J Agric Food Chem. 55, 5942-5947Link to the document url. peer-reviewed article