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Detection Image Detection method details

Name:

 QL-CON-35S166-F/hpt166-R

Description:

 Qualitative real-time PCR (TaqMan) method for detection of the junction between the CaMV 35S promoter and the hpt gene (Reiting et al., 2010).

Comment:

 Target is the junction region between the Cauliflower mosaic virus (CaMV) 35S promoter (P-35S) and the hygromycin phophotransferase II (hpt) gene.

Validation:

 unknown

Standardisation:

 none

Related Methods:

Type:

 construct-specific

Target GMO names:
Kefeng6

  • Oligonucleotides:

  • Forward Primer

  • Name:

    35S166-F

  • Sequence:

    GCTGAAATCACCAGTCTCTCTCTACA

  • Size:

    26

  • Reverse Primer

  • Name:

    hpt166-R

  • Sequence:

    TGAGTTCAGGCTTTTTCATATCTCAT

  • Size:

    26

  • Probe

  • Name:

    35S166-Tm

  • Sequence:

    TATCTCTCTCGAGCTTTCGCAGATCCGG

  • Size:

    28

  • Amplicon:

  • Sequence:

  • Size:

    90

Documents

Citation Type Local copy
Sampling and analysis guidelines for the detection of genetic modifications in riceLink to the document url. PCR Protocol Download PDF pdf
Reiting R, Grohmann L, Maede D (2010) A testing cascade for the detection of genetically modified rice by real-time PCR in food and its application for detection of an unauthorized rice line similar to KeFeng6. J. Verbr. Lebensm. 5:185-188Link to the document url. peer-reviewed article Download PDF pdf
Protocol QL-CON-35S166-F/hpt166-R PCR Protocol Download PDF pdf