Detection method details
Name: |
QT-TAX-ZM-004 |
Description: |
Quantitative real-time PCR (TaqMan) for detection of maize aldolase 1 (ald1) gene (EU-RL GMFF GMOMETHODS database as of July 14, 2016).
Estimated number of target copies per haploid genome (Jacchia et al. 2018): 2 |
Comment: |
Target the aldolase 1 (ald1) gene from Zea mays.
This assay is NOT recommended for GMO quantitative analysis because of a secondary target with mismatches in the primers and probe annealing region. |
Validation: |
ring trial validation |
Standardisation: |
EU reference method |
Related Methods: |
Type: |
taxon-specific |
Target Species name: |
Zea mays |
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Oligonucleotides:
Forward Primer
Name:
Aldolase primer FSequence:
AGGGAGGACGCCTCCCTSize:
17
Reverse Primer
Name:
Aldolase primer RSequence:
ACCCTGTACCAGAAGACCAAGGSize:
22
Probe
Name:
Aldolase probeSequence:
TGAGGACATCAACAAAAGGCTTGCCASize:
26
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Amplicon:
Sequence:
agggaggacgcctccctccttgaggacatcaacaaaaggcttgccatccttggtcttctggtacagggtSize:
69