Detection method details
Name: |
QT-CON-00-008 |
Description: |
Quantitative real-time PCR (TaqMan) method for detection of maize event GA21 (Shindo et al., 2002) |
Comment: |
Target is the junction region between an optimized transit peptide sequence (OTP) and point mutated epsps gene (mEPSPS) from maize |
Validation: |
ring trial validation |
Standardisation: |
ISO standard |
Related Methods: |
QT-TAX-ZM-006 |
Type: |
construct-specific |
Target GMO names: |
DAS44406, FG72, GA21, GHB614, GHB811
|
-
Oligonucleotides:
Forward Primer
Name:
GA21 3-5'Sequence:
GAAGCCTCGGCAACGTCASize:
18
Reverse Primer
Name:
GA21 3-3'Sequence:
ATCCGGTTGGAAAGCGACTTSize:
20
Probe
Name:
GA21-2-TaqSequence:
AAGGATCCGGTGCATGGCCGSize:
20
-
Amplicon:
Sequence:
gaagcctcggcaacgtcannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnaagtcgctttccaaccggatSize:
133
Documents
Citation | Type | Local copy |
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