Detection method details
Name: |
QT-ELE-00-004 |
Description: |
Quantitative real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter (ISO 21570 Annex B.1) |
Comment: |
the target is the 35S promoter (P-35S) from Cauliflower mosaic virus |
Validation: |
ring trial validation |
Standardisation: |
ISO standard |
Related Methods: |
Type: |
element-specific |
Target DNA element: |
E-35S-CaMV, P-Cauliflower mosaic virus, P-scp1-SYNTH
|
-
Oligonucleotides:
Forward Primer
Name:
35S-FSequence:
GCCTCTGCCGACAGTGGTSize:
18
Reverse Primer
Name:
35S-RSequence:
AAGACGTGGTTGGAACGTCTTCSize:
22
Probe
Name:
35S-TMPSequence:
CAAAGATGGACCCCCACCCACGSize:
22
-
Amplicon:
Sequence:
gcctctgccgacagtggtcccaaagatggacccccacccacgaggagcatcgtggaaaaagaagacgttccaaccacgtcttSize:
82
Documents
Citation | Type | Local copy |
---|---|---|
ISO21570:2005(E) Annex B (informative) | EU reference method protocol | |
EU-RL GMFF GMOMETHODS database; QT-ELE-00-004 | EU reference method protocol |