Detection method details
Name: |
QT-TAX-ZM-002 |
Description: |
Quantitative real-time PCR (TaqMan) method for detection of the maize high-mobility-group A (hmgA) gene (ISO 21570).
Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1 |
Comment: |
Target is the endogenous high-mobility-group A (hmgA) gene from Zea mays. This assay is recommended for quantitative GMO analysis. |
Validation: |
ring trial validation |
Standardisation: |
ISO standard |
Related Methods: |
Type: |
taxon-specific |
Target Species name: |
Zea mays |
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Oligonucleotides:
Forward Primer
Name:
ZM1-FSequence:
TTGGACTAGAAATCTCGTGCTGASize:
23
Reverse Primer
Name:
ZM1-RSequence:
GCTACATAGGGAGCCTTGTCCTSize:
22
Probe
Name:
Probe ZM1Sequence:
CAATCCACACAAACGCACGCGTASize:
23
-
Amplicon:
Sequence:
ttggactagaaatctcgtgctgannnnnnnnnntacgcgtgcgtttgtgtggattgnaggacaaggctccctatgtagcSize:
79