Detection method details
Name: |
QL-ELE-00-015 |
Description: |
Qualitative real-time PCR (TaqMan) method for detection of Figwort mosaic virus 34S promoter (ISO/TS 21569-5). |
Comment: |
Target is the Figwort mosaic virus 34S promoter (P-FMV), which in some GMOs is indicated as Figwort mosaic virus 34S enhancer (E-FMV). |
Validation: |
ring trial validation |
Standardisation: |
ISO standard |
Related Methods: |
Type: |
element-specific |
Target DNA element: |
E-FMV, P-34S FMV
|
-
Oligonucleotides:
Forward Primer
Name:
pFMV-FSequence:
CAAAATAACGTGGAAAAGAGCTSize:
22
Reverse Primer
Name:
pFMV-RSequence:
TCTTTTGTGGTCGTCACTGCSize:
20
Probe
Name:
Probe pFMVSequence:
CTGACAGCCCACTCACTAATGCSize:
22
-
Amplicon:
Sequence:
caaaataacgtggaaaagagctnnnctgacagcccactcactaatgcnnnnnnnnnnngcagtgacgaccacaaaagaSize:
78
Documents
Citation | Type | Local copy |
---|---|---|
EU-RL GMFF GMOMETHODS database; QL-ELE-00-015 | EU reference method protocol |