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Detection Image Detection method details

Name:

 QL-ELE-00-008

Description:

 Qualitative real-time PCR (TaqMan) method for detection of nopaline synthase promoter (ISO/TS 21569-4)

Comment:

 Target is the nopaline synthase promoter (P-nos) from Agrobacterium tumefaciens

Validation:

 ring trial validation

Standardisation:

 ISO standard

Related Methods:

Type:

 element-specific

Target DNA element:
P-nos-RHIRD

  • Oligonucleotides:

  • Forward Primer

  • Name:

    p-nos-F1

  • Sequence:

    AAGCACATACGTCAGAAACCATTATT

  • Size:

    26

  • Reverse Primer

  • Name:

    p-nos-R

  • Sequence:

    TCAGTGGAGCATTTTTGACAAGAA

  • Size:

    24

  • Probe

  • Name:

    p-nos-Tm

  • Sequence:

    CGCGTTCAAAAGTCGCCTAAGGTCAC

  • Size:

    26

  • Amplicon:

  • Sequence:

    aagcacatacgtcagaaaccattattncgcgttcaaaagtcgcctaaggtcacnnnnnnnnnnnnnnnnnttcttgtcaaaaatgctccactga

  • Size:

    94

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QL-ELE-00-008Link to the document url. EU reference method protocol