Detection method details
Name: |
QT-CON-00-002 |
Description: |
Quantitative real-time PCR (TaqMan) method for detection of soybean event GTS 40-3-2 (Hird et al., 2003). |
Comment: |
Target is the junction region between the chloroplast transit peptide (CTP) sequence from Petunia hybrida epsps gene and the CP4 epsps gene from Agrobacterium tumefaciens |
Validation: |
ring trial validation |
Standardisation: |
national standard |
Related Methods: |
QT-TAX-GM-004 |
Type: |
construct-specific |
Target GMO names: |
GTS 40-3-2
|
-
Oligonucleotides:
Forward Primer
Name:
RR-FSequence:
GGATTTCAGCATCAGTGGCTACASize:
23
Reverse Primer
Name:
RR-RSequence:
CCGGAAAGGCCAGAGGATSize:
18
Probe
Name:
RR soyaSequence:
CCGGCTGCTTGCACCGTGAAGSize:
21
-
Amplicon:
Sequence:
ggatttcagcatcagtggctacannnnnnnnncttcacggtgcaagcagccggnnnnnnnnnnnnnnnnnatcctctggcctttccggSize:
88
Documents
Citation | Type | Local copy |
---|---|---|
Hird H., Powell J., Johnson M.L., Oehlschlager S.; Determination of percentage of RoundUp Ready soya in soya flour using real-time polymerase chain reaction: interlaboratory study J AOAC Int 86:66-71 (2003) | peer-reviewed article | |
PCR reactions set up and amplification conditions Online Publication (2010); QT-CON-00-002 | EU reference method protocol | |
EU-RL GMFF GMOMETHODS database; QT-CON-00-002 | EU reference method protocol |