Detection method details
Name: |
QT-EVE-BN-013 |
Description: |
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape event LBFLFK locus2 (EU-RL GMFF GMOMETHODS database as of October 12, 2022) |
Comment: |
Target is the 5' integration border region (IBR) between the insert of oilseed rape event LBFLFK Locus2 and the oilseed rape host genome. |
Validation: |
ring trial validation |
Standardisation: |
EU reference method |
Related Methods: |
QT-TAX-BN-001 |
Type: |
event-specific |
Target GMO name: |
LBFLFK |
-
Oligonucleotides:
Forward Primer
Name:
rightFST_ev1_loc2-f4Sequence:
GATTGGTAATATGTAAATAACGGGATCCSize:
28
Reverse Primer
Name:
rightFST_ev1_loc2-r1Sequence:
GCGAATTTGGCCTGTAGACCSize:
20
Probe
Name:
rightFST_ev1_loc2-s1Sequence:
CATCACACCAAAAGTTAGGCCCGAASize:
25
-
Amplicon:
Sequence:
gattggtaat atgtaaataa cgggatccnn nnnnnnnnnn nnnnnnnnnc atcacaccaa aagttaggcc cgaannnnnn nnnnnnnnnn nnnnnnnnnn nnnggtctac aggccaaatt cgcSize:
123
Documents
Citation | Type | Local copy |
---|---|---|
EU-RL GMFF GMOMETHODS database; QT-EVE-BN-013 | EU reference method protocol |