Detection method details
Name: |
QT-CON-00-007 |
Description: |
Quantitative real-time PCR (TaqMan) method for detection of maize event Bt176 (Shindo et al., 2002) |
Comment: |
Target is the junction region between a synthetic cryIA(b) gene and the phospho-enol-pyruvate carboxylase (PEPC) intron N.9 |
Validation: |
ring trial validation |
Standardisation: |
ISO standard |
Related Methods: |
QT-TAX-ZM-006 |
Type: |
construct-specific |
Target GMO names: |
B827, Bt176
|
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Oligonucleotides:
Forward Primer
Name:
E176 2-5'Sequence:
TGTTCACCAGCAGCAACCAGSize:
20
Reverse Primer
Name:
E176 2-3'Sequence:
ACTCCACTTTGTGCAGAACAGATCTSize:
25
Probe
Name:
E176-TaqSequence:
CCGACGTGACCGACTACCACATCGASize:
25
-
Amplicon:
Sequence:
tgttcaccagcagcaaccagnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnagatctgttctgcacaaagtggagtSize:
100