Detection method details
Name: |
QL-ELE-RERIO 2206/RERIO 2207 |
Description: |
Qualitative conventional PCR method for detection of the modified red fluorescent protein (DsRed2) marker gene (Rehbein & Bogerd, 2007). |
Comment: |
Target is the modified red fluorescent protein (DsRed2) gene from Discosoma sp. (sequence in pDsRed2 vector of Clontech, see GenBank EA057759.1). |
Validation: |
unknown |
Standardisation: |
none |
Related Methods: |
Type: |
element-specific |
Target DNA element: |
CS-DsRed2 fluorescent protein
|
-
Oligonucleotides:
Forward Primer
Name:
RERIO 2206Sequence:
ACAACACCGTGAAGCTGAAGGTGACCAAGSize:
29
Reverse Primer
Name:
RERIO 2207Sequence:
GGTGTAGTCCTCGTTGTGGGAGGTGATGTCSize:
30
-
Amplicon:
Sequence:
acaacaccgtgaagctgaaggtgaccaagggcggccccctgcccttcgcctgggacatcctgtccccccagttccagtacggctccaaggtgtacgtgaagcaccccgccgacatccccgactacaagaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggcgaccgtgacccaggactcctccctgcaggacggctgcttcatctacaaggtgaagttcatcggcgtgaacttcccctccgacggccccgtgatgcagaagaagaccatgggctgggaggcctccaccgagcgcctgtacccccgcgacggcgtgctgaagggcgagacccacaaggccctgaagctgaaggacggcggccactacctggtggagttcaagtccatctacatggccaagaagcccgtgcagctgcccggctactactacgtggacgccaagctggacatcacctcccacaacgaggactacaccSize:
506
Documents
Citation | Type | Local copy |
---|---|---|
H. Rehbein & J. Bogerd (2007) Identification of Genetically Modified Zebrafish (Danio rerio) by Protein- and DNA-Analysis. J. Verbr. Lebensm. 2:122-125 | peer-reviewed article |