Detection method details
Name: |
QT-TAX-ZM-003 |
Description: |
Quantitative real-time PCR (TaqMan) method for detection of maize alcohol dehydrogenase 1 (adh1) gene (EU-RL GMFF GMOMETHODS database as of October 01, 2014) |
Comment: |
Target is the alcohol dehydrogenase 1 (adh1) gene from Zea mays |
Validation: |
ring trial validation |
Standardisation: |
EU reference method |
Related Methods: |
Type: |
taxon-specific |
Target Species name: |
Zea mays |
-
Oligonucleotides:
Forward Primer
Name:
ADH-FF3Sequence:
CGTCGTTTCCCATCTCTTCCTCCTSize:
24
Reverse Primer
Name:
ADH-RR4Sequence:
CCACTCCGAGACCCTCAGTCSize:
20
Probe
Name:
ADH1-MDOSequence:
AATCAGGGCTCATTTTCTCGCTCCTCASize:
27
-
Amplicon:
Sequence:
cgtcgtttcccatctcttcctcctttagagctaccactatataaatcagggctcattttctcgctcctcacaggctcatctcgctttggatcgattggtttcgtaactggtgaaggactgagggtctcggagtggSize:
135
Documents
Citation | Type | Local copy |
---|---|---|
EU-RL GMFF GMOMETHODS database; QT-TAX-ZM-003 | EU reference method protocol |