Detection method details
Name: |
QL-TAX-ZM-002 |
Description: |
Qualitative real-time PCR (SYBRGreen) method for detection of maize alcoholdeydrogenase 1 gene (Barbau-Piednoir et al., 2014) |
Comment: |
Target is the endogenous alcohol dehydrogenase 1 (adh1) gene of Zea mays |
Validation: |
ring trial validation |
Standardisation: |
unknown |
Related Methods: |
Type: |
taxon-specific |
Target Species name: |
Zea mays |
-
Oligonucleotides:
Forward Primer
Name:
ADH_alt FwdSequence:
TCTCTTCCTCCTTTAGAGCTACCACTASize:
27
Reverse Primer
Name:
ADH_alt RevSequence:
AATCGATCCAAAGCGAGATGASize:
21
-
Amplicon:
Sequence:
tctcttcctcctttagagctaccactannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntcatctcgctttggatcgattSize:
83
Documents
Citation | Type | Local copy |
---|---|---|
Barbau-Piednoir, E., Stragier, P., Roosens, N., Mazzara, M., Savini, C., Van den Eede, G., Van den Bulcke, M. (2014). Inter-laboratory testing of GMO detection by combinatory SYBR®Green PCR screening (CoSYPS). Food Analytical Methods. 7, 1719-1728. | peer-reviewed article | |
EU-RL GMFF GMOMETHODS database; QL-TAX-ZM-002 | EU reference method protocol |