Detection method details
Name: |
QL-ELE-00-016 |
Description: |
Qualitative real-time PCR (TaqMan) method for detection of the element coding for synthetic cry1Ab/Ac genes (ISO/TS 21569-6). |
Comment: |
Targets are synthetic cry1Ab, cry1Ac or cry1Ab/Ac fusion genes derived from Bacillus thuringiensis. |
Validation: |
ring trial validation |
Standardisation: |
ISO standard |
Related Methods: |
Type: |
element-specific |
Target DNA element: |
CS-cry1Ab_1Ac_tr-SYNTH, CS-cry1Ac-BACTU, CS-cry1Ab-BACTU
|
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Oligonucleotides:
Forward Primer
Name:
Bt-F1(mod)Sequence:
GAGGAAATGCGTATTCAATTCAACSize:
24
Reverse Primer
Name:
Bt-RSequence:
TTCTGGACTGCGAACAATGGSize:
20
Probe
Name:
Bt-PSequence:
ACATGAACAGCGCCTTGACCACAGCSize:
25
-
Amplicon:
Sequence:
gaggaaatgcgtattcaattcaacgacatgaacagcgccttgaccacagctatcccattgttcgcagtccagaaSize:
74