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Detection method details
Name: |
QT-TAX-GM-007 |
Description: |
Quantitative real-time PCR (TaqMan) method for detection of soybean lectin gene |
Comment: |
target is the endogenous lectin (Le1) gene of soybean |
Validation: |
ring trial validation |
Standardisation: |
ISO standard |
Related Methods: |
Type: |
taxon-specific |
Target Species name: |
Glycine max |
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Oligonucleotides:
Forward Primer
Name:
Le1n02-5'Sequence:
GCCCTCTACTCCACCCCCASize:
19
Reverse Primer
Name:
Le1n02-3'Sequence:
GCCCATCTGCAAGCCTTTTTSize:
20
Probe
Name:
Le1-TaqSequence:
AGCTTCGCCGCTTCCTTCAACTTCACSize:
26
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Amplicon:
Sequence:
gccctctactccacccccannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnagcttcgccgcttccttcaacttcacnnnnnnnnnnnnnnnnnnaaaaaggcttgcagatgggcSize:
118