Detection method details
Name: |
QL-ELE-00-021 |
Description: |
Qualitative real-time PCR (SYBRGreen) method for detection of CS-pat-STRVR (Barbau-Piednoir et al., 2014) |
Comment: |
Target is CS-pat-STRVR from Streptomyces viridochromogenes |
Validation: |
ring trial validation |
Standardisation: |
unknown |
Related Methods: |
QL-TAX-BN-003, QL-TAX-GM-001, QL-TAX-ZM-002 |
Type: |
element-specific |
Target DNA element: |
CS-pat-STRVR
|
-
Oligonucleotides:
Forward Primer
Name:
Pat-Pat FwdSequence:
CCGCGGTTTGTGATATCGTTSize:
20
Reverse Primer
Name:
Pat-Pat RevSequence:
TCTTGCAACCTCTCTAGATCATCAASize:
25
-
Amplicon:
Sequence:
ccgcggtttgtgatatcgttnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnttgatgatctagagaggttgcaagaSize:
109
Documents
| Citation | Type | Local copy |
|---|---|---|