Detection method details
Name: |
QL-ELE-00-012 |
Description: |
Qualitative real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter (partim P-35S).
Part of the qualitative duplex real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter and nopaline synthase terminator (ISO 21569 AMD1) |
Comment: |
Target is the 35S promoter (P-35S) from Cauliflower mosaic virus. For details of the detection of nopaline synthase terminator (partim T-nos) by this duplex real-time PCR method refer to QL-ELE-00-013 |
Validation: |
ring trial validation |
Standardisation: |
ISO standard |
Related Methods: |
QL-ELE-00-013 |
Type: |
element-specific |
Target DNA element: |
E-35S-CaMV, P-Cauliflower mosaic virus, P-scp1-SYNTH
|
-
Oligonucleotides:
Forward Primer
Name:
35S-FTMSequence:
GCCTCTGCCGACAGTGGTSize:
18
Reverse Primer
Name:
35S-RTMSequence:
AAGACGTGGTTGGAACGTCTTCSize:
22
Probe
Name:
35S-TMP-FAMSequence:
CAAAGATGGACCCCCACCCACGSize:
22
-
Amplicon:
Sequence:
gcctctgccgacagtggtcccaaagatggacccccacccacgaggagcatcgtggaaaaagaagacgttccaaccacgtcttSize:
82
Documents
Citation | Type | Local copy |
---|---|---|
Waiblinger H.-U., Ernst B., Anderson A., Pietsch K.; Validation and collaborative study of a P35S and T-nos duplex real-time PCR screening method to detect genetically modified organisms in food products Eur. Food Res. Technol. 226:1221-1228 (2008) | peer-reviewed article | |
EU-RL GMFF GMOMETHODS database; QL-ELE-00-012 | EU reference method protocol |