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Detection Image Detection method details

Name:

 QL-CON-00-013

Description:

 Qualitative real-time PCR (TaqMan) method for detection of the junction between the maize ubiquitin promoter and the modified cry1Ab/1Ac genes (Grohmann et al.,2015).

Comment:

 Target is the junction region between the ubiquitin promoter (P-ubi) sequence from maize (Zea mays) and the cry1Ab/Ac genes from Bacillus thuringiensis.

Validation:

 ring trial validation

Standardisation:

 EU reference method

Related Methods:

Type:

 construct-specific

Target GMO names:
3006-210-23, 4114, DAS1507, DAS59122, DAS59132, KMD1, Kefeng6

  • Oligonucleotides:

  • Forward Primer

  • Name:

    pubi-F2

  • Sequence:

    ATTTGCTTGGTACTGTTTCTTTTGTC

  • Size:

    26

  • Reverse Primer

  • Name:

    cry-rr-R

  • Sequence:

    TTGTTGTCCATGGATCCTCTAGAGT

  • Size:

    25

  • Probe

  • Name:

    pubi-T2

  • Sequence:

    ACCCTGTTGTTTGGTGTTACTTCTGCA

  • Size:

    27

  • Amplicon:

  • Sequence:

    atttgcttggtactgtttcttttgtcgatgctcaccctgttgtttggtgttacttctgcaggtcgactctagaggatccatggacaacaa

  • Size:

    90

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QL-CON-00-013Link to the document url. EU reference method protocol
Grohmann L., Reiting R., Maede D., Uhlig S., Simon K., Frost K., Jit Randhawa G., Zur K. Collaborative trial validation of cry1Ab/Ac and Pubi-cry TaqMan-based real-time PCR assays for detection of DNA derived from genetically modified Bt plant products. Accred Qual Assur 20:85-96 (2015)Link to the document url. peer-reviewed article