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Detection method details
Name: |
QT-TAX-GM-001 |
Description: |
Quantitative real-time PCR (TaqMan) method for detection of soybean lectin gene |
Comment: |
Target is the endogenous lectin (Le1) gene of Glycine max |
Validation: |
ring trial validation |
Standardisation: |
ISO standard |
Related Methods: |
Type: |
taxon-specific |
Target Species name: |
Glycine max |
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Oligonucleotides:
Forward Primer
Name:
Lectin-FSequence:
TCCACCCCCATCCACATTTSize:
19
Reverse Primer
Name:
Lectin-RSequence:
GGCATAGAAGGTGAAGTTGAAGGASize:
24
Probe
Name:
Lectin-TMPSequence:
AACCGGTAGCGTTGCCAGCTTCGSize:
23
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Amplicon:
Sequence:
tccacccccatccacatttnnnnnnnnnnaaccggtagcgttgccagcttcgnnnnntccttcaacttcaccttctatgccSize:
81