Detection method details
Name: |
QT-TAX-GH-019 |
Description: |
Quantitative real-time PCR (Taqman) method for detection of cotton alcohol dehydrogenase C gene (EU-RL GMFF GMOMETHODS database as of November 1, 2018).
Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1 |
Comment: |
Target is the endogenous alcohol dehydrogenase C (AdhC) gene of cotton.
This assay is recommended for GMO quantitative analysis. |
Validation: |
ring trial validation |
Standardisation: |
EU reference method |
Related Methods: |
Type: |
taxon-specific |
Target Species name: |
Gossypium hirsutum |
-
Oligonucleotides:
Forward Primer
Name:
KVM157Sequence:
CACATGACTTAGCCCATCTTTGCSize:
23
Reverse Primer
Name:
KVM158Sequence:
CCCACCCTTTTTTGGTTTAGCSize:
21
Probe
Name:
TM012Sequence:
TGCAGGTTTTGGTGCCACTGTGAATGSize:
26
-
Amplicon:
Sequence:
cacatgacttagcccatctttgcntgcaggttttggtgccactgtgaatgnngctaaaccaaaaaagggtgggSize:
73
Documents
Citation | Type | Local copy |
---|---|---|
Jacchia S., Kagkli D.F., Lievens A., Angers-Loustau A., Savini C., Emons H., Mazzara M. (2018) Identification of single target taxon-specific reference assays for the most commonly genetically transformed crops using digital droplet PCR. Food Control 93,191-200 | peer-reviewed article | |
EU-RL GMFF GMOMETHODS database; QT-TAX-GH-019 | EU reference method protocol |