Detection method details
Name: |
QT-TAX-FatA primer1/FatA primer2 |
Description: |
Quantitative real-time PCR (TaqMan) method for the detection of oilseed rape (Monsanto, 2004, A recommended procedure for real-time quantitative TaqMan PCR for Roundup Ready canola RT73).
Estimated number of target copies per haploid genome (Jacchia et al. 2018): 2 |
Comment: |
Target is the endogenous acyl-acyl carrier protein thioesterase (FatA) gene of Brassica napus.
This assay is NOT recommended for GMO quantitative analysis because of a secondary target with mismatches in the primers and probe annealing region. |
Validation: |
in-house validation |
Standardisation: |
none |
Related Methods: |
Type: |
taxon-specific |
Target Species name: |
Brassica napus |
-
Oligonucleotides:
Forward Primer
Name:
FatA primer1Sequence:
GGTCTCTCAGCAAGTGGGTGATSize:
22
Reverse Primer
Name:
FatA primer2Sequence:
TCGTCCCGAACTTCATCTGTAASize:
22
Probe
Name:
FatA probeSequence:
ATGAACCAAGACACAAGGCGGCTTCASize:
26
-
Amplicon:
Sequence:
GGTCTCTCAGCAAGTGGGTGATGATGAACCAAGACACAAGGCGGCTTCAAAGAGTTACAGATGAAGTTCGGGACGASize:
76