Detection method details
Name: |
QT-EVE-BN-002 |
Description: |
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape event Ms8 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) |
Comment: |
Target is the 3' integration border region (IBR) between the insert of oilseed rape event Ms8 and the oilseed rape host genome |
Validation: |
ring trial validation |
Standardisation: |
EU reference method |
Related Methods: |
QT-TAX-BN-012 |
Type: |
event-specific |
Target GMO name: |
Ms8 |
-
Oligonucleotides:
Forward Primer
Name:
KVM085Sequence:
GTTAGAAAAAGTAAACAATTAATATAGCCGGSize:
31
Reverse Primer
Name:
HCA048Sequence:
GGAGGGTGTTTTTGGTTATCSize:
20
Probe
Name:
TM011Sequence:
AATATAATCGACGGATCCCCGGGAATTCSize:
28
-
Amplicon:
Sequence:
gttagaaaaagtaaacaattaatatagccggnnnnnnnnnnnnnnnnnnnnaatataatcgacggatccccgggaattcnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnngataaccaaaaacaccctccSize:
130
Documents
Citation | Type | Local copy |
---|---|---|
Mazzara M., Bogni A., Savini C., Van Den Eede G.; Event-specific Method for the Quantification of Oilseed Rape Line Ms8 Using Real-time PCR - Validation Report and Protocol- Seeds Sampling and DNA Extraction of Oilseed Rape Online Publication (2007) | EU reference method protocol | |
EU-RL GMFF GMOMETHODS database; QT-EVE-BN-002 | EU reference method protocol |