Detection method details
Name: |
QT-TAX-wx012-5'/wx012-3' |
Description: |
Quantitative real-time PCR (TaqMan) method for detection of the common wheat waxy-D1 gene (Iida et al., 2005) |
Comment: |
Target is the waxy-D1 gene coding for the granule-bound starch synthase from common wheat (Triticum aestivum) |
Validation: |
in-house validation |
Standardisation: |
none |
Related Methods: |
Type: |
taxon-specific |
Target Species name: |
Triticum aestivum |
-
Oligonucleotides:
Forward Primer
Name:
wx012-5'Sequence:
GTCGCGGGAACAGAGGTGTSize:
19
Reverse Primer
Name:
wx012-3'Sequence:
GGTGTTCCTCCATTGCGAAASize:
20
Probe
Name:
wx012-TSequence:
CAAGGCGGCCGAAATAAGTTGCCSize:
23
-
Amplicon:
Sequence:
GTCGCGGGAACAGAGGTGTTCAAGGCGGCCGAAATAAGTTGCCGCCTGCGGCGGAATCGCCACCCACCGTGAAGTTCACCGTTTCGCAATGGAGGAACACCSize:
102