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Detection Image Detection method details

Name:

 QT-EVE-ZM-038

Description:

 Quantitative real-time PCR (TaqMan) method for detection of maize event DP910521 (EU-RL GMFF GMOMETHODS database as of June 10, 2024).

Comment:

 Target is the 5' integration border region (IBR) between the insert of maize event DP910521 and the maize host genome

Validation:

 ring trial validation

Standardisation:

 EU reference method

Related Methods:

 QT-TAX-ZM-008

Type:

 event-specific

Target GMO name:

 DP910521

  • Oligonucleotides:

  • Forward Primer

  • Name:

    PHN201324

  • Sequence:

    CTCTTGACACTTTGTATTGGTGCTC

  • Size:

    25

  • Reverse Primer

  • Name:

    PHN165631

  • Sequence:

    CATAGTAACCGTGAGCGCTTCA

  • Size:

    22

  • Probe

  • Name:

    PHN201325

  • Sequence:

    TTGGGCTCAAGAGGGTA

  • Size:

    17

  • Amplicon:

  • Sequence:

    ctcttgacactttgtattggtgctcttgggctcaagagggtannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntgaagcgctcacggttactatg

  • Size:

    108

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QT-EVE-ZM-038Link to the document url. EU reference method protocol