Detection method details
Name: |
QL-ELE-00-024 |
Description: |
Qualitative real-time PCR (TaqMan) method for detection of the terminator of the pea ribulose-1,5-bisphosphate carboxylase small subunit (rbcS) E9 gene (EU-RL GMFF GMOMETHODS database as of January 20, 2017). |
Comment: |
Target is the E9 (T-E9) terminator from pea (Pisum sativum). |
Validation: |
ring trial validation |
Standardisation: |
EU reference method |
Related Methods: |
Type: |
element-specific |
Target DNA element: |
T-rbcS_E9-PEA
|
-
Oligonucleotides:
Forward Primer
Name:
T-E9-FSequence:
TGAGAATGAACAAAAGGACCATATCASize:
26
Reverse Primer
Name:
T-E9-RSequence:
TTTTTATTCGGTTTTCGCTATCGSize:
23
Probe
Name:
T-E9-PSequence:
TCATTAACTCTTCTCCATCCATTTCCATTTCACAGTSize:
36
-
Amplicon:
Sequence:
tgagaatgaacaaaaggaccatatcattcattaactcttctccatccatttccatttcacagttcgatagcgaaaaccgaataaaaaSize:
87