Help icon

Detection Image Detection method details

Name:

 QL-TAX-CP-001

Description:

 Qualitative conventional PCR method for detection of the chymopapain (chy) gene from papaya (EU-RL GMFF GMOMETHODS database as of May 23, 2016)

Comment:

 Target is the endogenous chymopapain (chy) gene from papaya (Carica papaya)

Validation:

 ring trial validation

Standardisation:

 EU reference method

Related Methods:

 QL-CON-00-012

Type:

 taxon-specific

Target Species name:

 Carica papaya

  • Oligonucleotides:

  • Forward Primer

  • Name:

    Chy-1F

  • Sequence:

    ATCTACAATCTTGCTAACCCTA

  • Size:

    22

  • Reverse Primer

  • Name:

    Chy-2R

  • Sequence:

    AGTCATCTTGAGAATAACCCAC

  • Size:

    22

  • Amplicon:

  • Sequence:

    atctacaatcttgctaaccctaaaatgacggtgcaagtagagtgaatgacaagatcttttatttagagagaaagaaacttgattgaaaagacaagctagtatataaacccatgcggatcctcccatttcccttcatccattcccactcttgagagttagctagtgttacaatggctacgatgagttcaatttcaaaaattattttcttggcaacatgtctcattattcatatgggtttgtcttctgctgatttttacaccgtgggttattctcaagatgact

  • Size:

    282

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QL-TAX-CP-001Link to the document url. EU reference method protocol