Detection method details
Name: |
QL-ELE-00-002 |
Description: |
Qualitative conventional PCR method for detection of neomycin phosphotransferase II gene (ISO 21569) |
Comment: |
Target is the neomycin phosphotransferase II (nptII) gene from Escherichia colinpt |
Validation: |
ring trial validation |
Standardisation: |
ISO standard |
Related Methods: |
Type: |
element-specific |
Target DNA element: |
CS-nptII-ECOLX, V-nptII-ECOLX
|
-
Oligonucleotides:
Forward Primer
Name:
APH2 shortSequence:
CTCACCTTGCTCCTGCCGAGASize:
21
Reverse Primer
Name:
APH2 reverseSequence:
CGCCTTGAGCCTGGCGAACAGSize:
21
-
Amplicon:
Sequence:
ctcaccttgctcctgccgagannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnctgttcgccaggctcaaggcgSize:
215
Documents
Citation | Type | Local copy |
---|---|---|
EU-RL GMFF GMOMETHODS database; QL-ELE-00-002 | EU reference method protocol | |
Foodstuffs - Methods of analysis for the detection of genetically modified organisms and derived products - Qualitative nucleic acid based methods ISO 21569:1-69 (2005) | other document |