Detection method details
Name: |
QL-EVE-Oxy-3F/Oxy-4R |
Description: |
Qualitative real-time PCR (TaqMan) method for detection of of oilseed rape event OXY-235 (Yang et al. 2008) |
Comment: |
Target is the 3' integration border region (IBR) between the insert of oilseed rape event OXY-235 and the oilseed rape host genome |
Validation: |
in-house validation |
Standardisation: |
none |
Related Methods: |
QT-TAX-BN-012 |
Type: |
event-specific |
Target GMO name: |
OXY-235 |
-
Oligonucleotides:
Forward Primer
Name:
Oxy-3FSequence:
CTAACTTTTGGTGTGATGATGCTGASize:
25
Reverse Primer
Name:
Oxy-4RSequence:
CGATAGATGGTGGTGTGAGTCTTGSize:
24
Probe
Name:
Oxy-pSequence:
AGCTGATGGCAAGTTAATCTCCCCGAAGTCGSize:
31
-
Amplicon:
Sequence:
ctaacttttggtgtgatgatgctgactggcaagttaatctagtttccggttatgaagcacggcgtgtcagctgatggcaagttaatctccccgaagtcgacaagactcacaccaccatctatcgSize:
124