Detection method details
Name: |
QT-CON-00-004 |
Description: |
Quantitative real-time PCR (TaqMan) method for detection of maize event MON810 (Shindo et al., 2002) |
Comment: |
Target is the junction region between the Intron 1 from the maize hsp70 gene (IVS-HSP) and a synthetic cry1A(b) gene |
Validation: |
ring trial validation |
Standardisation: |
ISO standard |
Related Methods: |
Type: |
construct-specific |
Target GMO names: |
DBN9936, MON80100, MON802, MON809, MON810
|
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Oligonucleotides:
Forward Primer
Name:
M810 2-5'Sequence:
GATGCCTTCTCCCTAGTGTTGASize:
22
Reverse Primer
Name:
M810 2-3'Sequence:
GGATGCACTCGTTGATGTTTGSize:
21
Probe
Name:
M810-TaqSequence:
AGATACCAAGCGGCCATGGACAACAASize:
26
-
Amplicon:
Sequence:
gatgccttctccctagtgttgannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnncaaacatcaacgagtgcatccSize:
113