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Detection Image Detection method details

Name:

 QT-TAX-GM-002

Description:

 Quantitative real-time PCR (TaqMan) method for detection of soybean lectin gene (ISO 21570).

Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1

Comment:

 Target is the endogenous lectin (Le1) gene from Glycine max. This assay is recommended for GMO quantitative analysis.

Validation:

 ring trial validation

Standardisation:

 ISO standard

Related Methods:

Type:

 taxon-specific

Target Species name:

 Glycine max

  • Oligonucleotides:

  • Forward Primer

  • Name:

    GM1-F

  • Sequence:

    CCAGCTTCGCCGCTTCCTTC

  • Size:

    20

  • Reverse Primer

  • Name:

    GM1-R

  • Sequence:

    GAAGGCAAGCCCATCTGCAAGCC

  • Size:

    23

  • Probe

  • Name:

    GM1

  • Sequence:

    CTTCACCTTCTATGCCCCTGACAC

  • Size:

    24

  • Amplicon:

  • Sequence:

    ccagcttcgccgcttccttcnncttcaccttctatgcccctgacacnnnnnggcttgcagatgggcttgccttc

  • Size:

    74

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QT-TAX-GM-002Link to the document url. EU reference method protocol
Jacchia S., Kagkli D.F., Lievens A., Angers-Loustau A., Savini C., Emons H., Mazzara M. (2018) Identification of single target taxon-specific reference assays for the most commonly genetically transformed crops using digital droplet PCR. Food Control 93,191-200Link to the document url. peer-reviewed article 31-10-2018
Foodstuffs - Methods of analysis for the detection of genetically modified organisms and derived products - Quantitative nucleic acid based methods ISO 21570:1-103 (2005)Link to the document url. other document