Detection method details
Name: |
QT-EVE-ZM-020 |
Description: |
Quantitative real-time PCR (TaqMan) method for detection of maize event MON810 (ISO/FDIS 21570) |
Comment: |
Target is the 5' integration border region (IBR) between the insert of maize event MON 810 and the maize host genome |
Validation: |
ring trial validation |
Standardisation: |
ISO standard |
Related Methods: |
QT-TAX-ZM-002 |
Type: |
event-specific |
Target GMO name: |
MON810 |
-
Oligonucleotides:
Forward Primer
Name:
Mail-F1Sequence:
TCGAAGGACGAAGGACTCTAACGTSize:
24
Reverse Primer
Name:
Mail-R1Sequence:
GCCACCTTCCTTTTCCACTATCTTSize:
24
Probe
Name:
mail-S2Sequence:
AACATCCTTTGCCATTGCCCAGCSize:
23
-
Amplicon:
Sequence:
tcgaaggacgaaggactctaacgtnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnaagatagtggaaaaggaaggtggcSize:
92
Documents
Citation | Type | Local copy |
---|---|---|
EU-RL GMFF GMOMETHODS database; QT-EVE-ZM-020 | EU reference method protocol | |
Foodstuffs - Methods of analysis for the detection of genetically modified organisms and derived products - Quantitative nucleic acid based methods ISO 21570:1-103 (2005) | other document |