Detection method details
Name: |
QL-ELE-P-FMV-F/P-FMV-R |
Description: |
Qualitative real-time PCR (TaqMan) method for detection of the P-FMV promoter (Debode et al., 2013) |
Comment: |
Target is the 34S promoter from FMV.
In some GMOs that only contain the E-FMV, this method also detects the GMO (e.g. soybean MON89788 and MON87705, canola MON88302 and cotton MON88913). |
Validation: |
in-house validation |
Standardisation: |
none |
Related Methods: |
Type: |
element-specific |
Target DNA element: |
P-34S FMV
|
-
Oligonucleotides:
Forward Primer
Name:
P-FMV-FSequence:
CGAAGACTTAAAGTTAGTGGGCATCTSize:
26
Reverse Primer
Name:
P-FMV-RSequence:
TTTTGTCTGGTCCCCACAASize:
19
Probe
Name:
P-FMV-PSequence:
TGAAAGTAATCTTGTCAACATCGAGCAGCTGGSize:
32
-
Amplicon:
Sequence:
CGAAGACTTAAAGTTAGTGGGCATCTTTGAAAGTAATCTTGTCAACATCGAGCAGCTGGCTTGTGGGGACCAGACAAAASize:
79