Detection method details
Name: |
QT-EVE-GH-006 |
Description: |
Quantitative real-time PCR (TaqMan) method for detection of cotton event GHB614 (EU-RL GMFF GMOMETHODS database as of January 13, 2014) |
Comment: |
Target is the 3' integration border region (IBR) between the insert of cotton event GHB614 and the cotton host genome |
Validation: |
ring trial validation |
Standardisation: |
EU reference method |
Related Methods: |
QT-TAX-GH-019 |
Type: |
event-specific |
Target GMO name: |
GHB614 |
-
Oligonucleotides:
Forward Primer
Name:
SHA007Sequence:
CAAATACACTTGGAACGACTTCGTSize:
24
Reverse Primer
Name:
SHA008Sequence:
GCAGGCATGCAAGCTTTTAAASize:
21
Probe
Name:
TM072Sequence:
CTCCATGGCGATCGCTACGTATCTAGAATTSize:
30
-
Amplicon:
Sequence:
caaatacacttggaacgacttcgtnnnnnnctccatggcgatcgctacgtatctagaattnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntttaaaagcttgcatgcctgcSize:
120
Documents
Citation | Type | Local copy |
---|---|---|
EU-RL GMFF GMOMETHODS database; QT-EVE-GH-006 | EU reference method protocol |