Detection method details
Name: |
QT-EVE-GM-011 |
Description: |
Quantitative real-time PCR (TaqMan) method for detection of soybean event CV127 (EU-RL GMFF GMOMETHODS database as of January 23, 2014) |
Comment: |
Target is the 3' integration border region (IBR) between the insert of soybean event CV127 and the soybean host genome |
Validation: |
ring trial validation |
Standardisation: |
EU reference method |
Related Methods: |
QT-TAX-GM-002 |
Type: |
event-specific |
Target GMO name: |
CV127 |
-
Oligonucleotides:
Forward Primer
Name:
SE-127-f4Sequence:
AACAGAAGTTTCCGTTGAGCTTTAAGACSize:
28
Reverse Primer
Name:
SE-127-r2Sequence:
CATTCGTAGCTCGGATCGTGTACSize:
23
Probe
Name:
SE-127-p3Sequence:
TTTGGGGAAGCTGTCCCATGCCCSize:
23
-
Amplicon:
Sequence:
aacagaagtttccgttgagctttaagacntttggggaagctgtcccatgcccnnnnnnnnnnnnngtacacgatccgagctacgaatgSize:
88
Documents
Citation | Type | Local copy |
---|---|---|
EU-RL GMFF GMOMETHODS database; QT-EVE-GM-011 | EU reference method protocol |