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Detection method details
Name: |
QL-ELE-P-SSuAra-F/P-SSuAra-R |
Description: |
Qualitative real-time PCR (TaqMan) method for detection of SSUAra promoter (ribulose 1.5-biphoshate carboxylase small subunit) of Arabidopsis thaliana (Debode et al., 2013) |
Comment: |
Target is the SSUAra promoter (P-rbcS) from Arabidopsis thaliana |
Validation: |
in-house validation |
Standardisation: |
none |
Related Methods: |
Type: |
element-specific |
Target DNA element: |
P-rbcS-ARATH
|
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Oligonucleotides:
Forward Primer
Name:
P-SSuAra-FSequence:
GGCCTAAGGAGAGGTGTTGAGASize:
22
Reverse Primer
Name:
P-SSuAra-RSequence:
CTCATAGATAACGATAAGATTCATGGAATTSize:
30
Probe
Name:
P-SSuAra-PSequence:
CCTTATCGGCTTGAACCGCTGGAATAASize:
27
-
Amplicon:
Sequence:
GGCCTAAGGAGAGGTGTTGAGACCCTTATCGGCTTGAACCGCTGGAATAATGCCACGTGGAAGATAATTCCATGAATCTTATCGTTATCTATGAGSize:
95