Detection method details
Name: |
QL-ELE-Vip3A-F/Vip3A-R |
Description: |
Qualitative real-time PCR (TaqMan) method for detection of the vip3A gene (Liang et al., 2013). |
Comment: |
Target is the vip3A gene from bacterium Bacillus thuringiensis strain AB88. |
Validation: |
in-house validation |
Standardisation: |
none |
Related Methods: |
Type: |
element-specific |
Target DNA element: |
CS-vip3Aa20-BACTU
|
-
Oligonucleotides:
Forward Primer
Name:
Vip3A-FSequence:
TCACCAAGAAGATGAAGACSize:
19
Reverse Primer
Name:
Vip3A-RSequence:
CTCTCCACCTTCTTCTTGSize:
18
Probe
Name:
Vip3A-PSequence:
GACCGCCAACTTCTACGACASize:
20
-
Amplicon:
Sequence:
-Size:
97
Documents
Citation | Type | Local copy |
---|---|---|
Liang et al., 2012 |