Detection method details
Name: |
QL-EVE-ZM-002 |
Description: |
Qualitative conventional PCR method for detection of maize event Bt10 (verified by the EU-RL GMFF in the context of Commission Decision 317/2005/EC) |
Comment: |
A target is not defined. However, this method specifically detects Bt10 maize an can discriminate between Bt10 and other maize events, also between Bt10 and Bt11. |
Validation: |
in-house validation |
Standardisation: |
none |
Related Methods: |
Type: |
event-specific |
Target GMO name: |
Bt10 maize |
-
Oligonucleotides:
Forward Primer
Name:
JSF5Sequence:
CACACAGGAGATTATTATAGGGTTACTCASize:
29
Reverse Primer
Name:
JSR5Sequence:
ACACGGAAATGTTGAATACTCATACTCTSize:
28
-
Amplicon:
Sequence:
cacacaggagattattatagggttactcannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnagagtatgagtattcaacatttccgtgtSize:
117
Documents
Citation | Type | Local copy |
---|---|---|
EU-RL GMFF GMOMETHODS database; QL-EVE-ZM-002 | EU reference method protocol |