Detection method details
Name: |
QT-TAX-zSSIIb-3F/zSSIIb-4R |
Description: |
Quantitative real-time PCR (TaqMan) method for detection of maize starch synthase IIb gene (Yang et al., 2005) |
Comment: |
Target is the endogenous starch synthase IIb (zSSIIb) gene from Zea mays. |
Validation: |
unknown |
Standardisation: |
unknown |
Related Methods: |
Type: |
taxon-specific |
Target Species name: |
Zea mays |
-
Oligonucleotides:
Forward Primer
Name:
zSSIIb-3FSequence:
CGGTGGATGCTAAGGCTGATGSize:
21
Reverse Primer
Name:
zSSIIb-4RSequence:
AAAGGGCCAGGTTCATTATCCTCSize:
23
Probe
Name:
zSSIIb-PSequence:
TAAGGAGCACTCGCCGCCGCATCTGSize:
25
-
Amplicon:
Sequence:
cggtggatgctaaggctgatgcagctccggctacagatgcggcggcgagtgctccttatgacagggaggataatgaacctggccctttSize:
88