Detection method details
Name: |
QL-ELE-00-025 |
Description: |
Qualitative real-time PCR (TaqMan) method for detection of the synthetic phosphinothricin acetyltransferase (pat) gene (BVL L00.00-154). EU-RL GMFF GMOMETHODS database as of December 23, 2016. |
Comment: |
Target is the synthetic phosphinothricin acetyltransferase (pat) gene from bacterium Streptomyces viridochromogenes.
For details of the detection of bar (partim bar) when applied as duplex real-time PCR method refer to QL-ELE-00-026 |
Validation: |
ring trial validation |
Standardisation: |
EU reference method |
Related Methods: |
Type: |
element-specific |
Target DNA element: |
CS-pat-STRVR
|
-
Oligonucleotides:
Forward Primer
Name:
pat-FSequence:
CGCGGTTTGTGATATCGTTAACSize:
22
Reverse Primer
Name:
pat-RSequence:
TCTTGCAACCTCTCTAGATCATCAASize:
25
Probe
Name:
pat-PSequence:
AGGACAGAGCCACAAACACCACAAGAGTGSize:
29
-
Amplicon:
Sequence:
cgcggtttgtgatatcgttaaccattacattgagacgtctacagtgaactttaggacagagccacaaacaccacaagagtggattgatgatctagagaggttgcaagaSize:
108