Detection method details
Name: |
QT-TAX-SL-002 |
Description: |
Quantitative real-time PCR (TaqMan) method for detection of the tomato LAT52 gene (ISO 21570 Amd1) |
Comment: |
target is the LAT52 gene from tomato (Solanum lycopersicum) |
Validation: |
ring trial validation |
Standardisation: |
ISO standard |
Related Methods: |
Type: |
taxon-specific |
Target Species name: |
Solanum lycopersicum |
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Oligonucleotides:
Forward Primer
Name:
LAT52 primer FSequence:
AGACCACGAGAACGATATTTGCSize:
22
Reverse Primer
Name:
LAT52 primer RSequence:
TTCTTGCCTTTTCATATCCAGACASize:
24
Probe
Name:
LAT52 probeSequence:
CTCTTTGCAGTCCTCCCTTGGGCTSize:
24
-
Amplicon:
Sequence:
agaccacgagaacgatatttgcnnnnnnnnnnnnnnnnnnagcccaagggaggactgcaaagagnnnntgtctggatatgaaaaggcaagaaSize:
92
Documents
Citation | Type | Local copy |
---|---|---|
EU-RL GMFF GMOMETHODS database; QT-TAX-SL-002 | EU reference method protocol | |
Yang L., Zhang H., Guo J., Pan L., Zhang D.; International Collaborative Study of the Endogenous Reference Gene LAT52 Used for Qualitative and Quantitative Analyses of Genetically Modified Tomato J. Agric. Food Chem. 56:3438-3443 (2008) | peer-reviewed article |