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Detection Image Detection method details

Name:

 QT-TAX-GM-020

Description:

 Quantitative real-time PCR (TaqMan) method for detection of soybean lectin (Le1) gene (EU-RL GMFF GMOMETHODS database as of January 22, 2014).

Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1

Comment:

 Target is the endogenous lectin (Le1) gene from Glycine max which is present as a single copy in the soybean genome. This assay is recommended for GMO quantitative analysis.

Validation:

 ring trial validation

Standardisation:

 EU reference method

Related Methods:

Type:

 taxon-specific

Target Species name:

 Glycine max

  • Oligonucleotides:

  • Forward Primer

  • Name:

    KVM 164

  • Sequence:

    CTTTCTCGCACCAATTGACA

  • Size:

    20

  • Reverse Primer

  • Name:

    KVM165

  • Sequence:

    TCAAACTCAACAGCGACGAC

  • Size:

    20

  • Probe

  • Name:

    TM021

  • Sequence:

    CCACAAACACATGCAGGTTATCTTGG

  • Size:

    26

  • Amplicon:

  • Sequence:

    ctttctcgcaccaattgacannnnnccacaaacacatgcaggttatcttggnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnngtcgtcgctgttgagtttga

  • Size:

    102

Documents

Citation Type Local copy
Jacchia S., Kagkli D.F., Lievens A., Angers-Loustau A., Savini C., Emons H., Mazzara M. (2018) Identification of single target taxon-specific reference assays for the most commonly genetically transformed crops using digital droplet PCR. Food Control 93,191-200Link to the document url. peer-reviewed article
EU-RL GMFF GMOMETHODS database; QT-TAX-GM-020Link to the document url. EU reference method protocol