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Detection method details
Name: |
QT-TAX-GM-009 |
Description: |
Quantitative real-time PCR (TaqMan) method for detection of soybean lectin (Le1) gene (EU-RL GMFF GMOMETHODS database as of January 22, 2014) |
Comment: |
Target is the endogenous lectin (Le1) gene from Glycine max |
Validation: |
ring trial validation |
Standardisation: |
EU reference method |
Related Methods: |
Type: |
taxon-specific |
Target Species name: |
Glycine max |
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Oligonucleotides:
Forward Primer
Name:
KVM164Sequence:
CACCTTTCTCGCACCAATTGACASize:
23
Reverse Primer
Name:
KVM165Sequence:
TCAAACTCAACAGCGACGACSize:
20
Probe
Name:
TM021Sequence:
CCACAAACACATGCAGGTTATCTTGGSize:
26
-
Amplicon:
Sequence:
cttctttctcgcaccaattgacannnnnccacaaacacatgcaggttatcttggnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnngtcgtcgctgttgagtttgaSize:
105
Documents
Citation | Type | Local copy |
---|---|---|
EU-RL GMFF GMOMETHODS database; QT-TAX-GM-009![]() |
EU reference method protocol |