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Detection Image Detection method details

Name:

 QT-EVE-ZM-040

Description:

 Quantitative real-time PCR (TaqMan) method for detection of maize event DAS1131 (EU-RL GMFF GMOMETHODS database as of January 15, 2025).

Comment:

 Target is the 5' integration border region (IBR) between the insert of maize event DAS1131 and the maize host genome.

Validation:

 ring trial validation

Standardisation:

 EU reference method

Related Methods:

 QT-TAX-ZM-008

Type:

 event-specific

Target GMO name:

 DAS1131

  • Oligonucleotides:

  • Forward Primer

  • Name:

    PHN201280

  • Sequence:

    CTAAGAGCTAAGATTGCGCGG

  • Size:

    21

  • Reverse Primer

  • Name:

    PHN201282

  • Sequence:

    TTCGGGCCTAACTTTTGGTG

  • Size:

    20

  • Probe

  • Name:

    PHN201284

  • Sequence:

    ACATATTTTTTGAGGATAACAGCA

  • Size:

    24

  • Amplicon:

  • Sequence:

    CTAAGAGCTAAGATTGCGCGGNNNNNNNNNNNNNNNNNNNNNNNNNNNACATATTTTTTGAGGATAACAGCANNNNNNCACCAAAAGTTAGGCCCGAA

  • Size:

    98

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QT-EVE-ZM-040Link to the document url. EU reference method protocol