Detection method details
Name: |
QT-EVE-GH-001a |
Description: |
Quantitative real-time PCR (TaqMan) method for detection of cotton event 281-24-236 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) |
Comment: |
Target is the 3' integration border region (IBR) between the insert of cotton event 281-24-236 and the cotton host genome |
Validation: |
ring trial validation |
Standardisation: |
EU reference method |
Related Methods: |
QT-TAX-GH-016, QT-TAX-GH-021 |
Type: |
event-specific |
Target GMO name: |
281-24-236 |
-
Oligonucleotides:
Forward Primer
Name:
281-f1Sequence:
CTCATTGCTGATCCATGTAGATTTCSize:
25
Reverse Primer
Name:
281-r2Sequence:
GGACAATGCTGGGCTTTGTGSize:
20
Probe
Name:
281-s2Sequence:
TTGGGTTAATAAAGTCAGATTAGAGGGAGACAASize:
33
-
Amplicon:
Sequence:
-Size:
111
Documents
Citation | Type | Local copy |
---|---|---|
Barbau-Piednoir, E., Stragier, P., Roosens, N., Mazzara, M., Savini, C., Van den Eede, G., Van den Bulcke, M. (2014). Inter-laboratory testing of GMO detection by combinatory SYBR®Green PCR screening (CoSYPS). Food Analytical Methods. 7, 1719-1728. | peer-reviewed article | |
EU-RL GMFF GMOMETHODS database; QT-EVE-GH-001a | EU reference method protocol |