Detection method details
Name: |
QT-CON-00-005 |
Description: |
Quantitative real-time PCR (TaqMan) method for detection of maize event T25 (Shindo et al., 2002) |
Comment: |
Target is the junction region between the phosphinothricin N-acetyltransferase (pat) gene from Streptomyces viridochromogenes and the Cauliflower mosaic virus 35S terminator (CaMV T-35S) |
Validation: |
ring trial validation |
Standardisation: |
ISO standard |
Related Methods: |
QT-TAX-ZM-006 |
Type: |
construct-specific |
Target GMO names: |
32316, 40416, 4114, 43A47, 676, 678, 680, A2704-12, A2704-21, A5547-127, A5547-35, DAS1507, DAS59122, DAS59132, DBN9004, DP62151, DP910521, Falcon GS 40/90, GU262, HCN10, HCR-1, Liberator, T120-7, T14, T25, T45, Topas 19/2
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Oligonucleotides:
Forward Primer
Name:
T25 1-5'Sequence:
GCCAGTTAGGCCAGTTACCCASize:
21
Reverse Primer
Name:
T25 1-3'Sequence:
TGAGCGAAACCCTATAAGAACCCTSize:
24
Probe
Name:
T25-2-TaqSequence:
TGCAGGCATGCCCGCTGAAATCSize:
22
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Amplicon:
Sequence:
gccagttaggccagttacccannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnagggttcttatagggtttcgctcaSize:
149