Detection method details
Name: |
QL-EVE-OS-KM2_for/KM1_rev |
Description: |
Qualitative real-time PCR (TaqMan) method for detection of rice event KMD1 |
Comment: |
Target is the 3' integration border region (IBR) between the insert of rice event KMD1 and the rice host genome.
The forward primer targets the pBn19 sequence from the insert, the reverse primer targets the rice host genome and the probe target the transition sequence between pBn19 and the host genome. |
Validation: |
in-house validation |
Standardisation: |
none |
Related Methods: |
Type: |
event-specific |
Target GMO name: |
KMD1 |
-
Oligonucleotides:
Forward Primer
Name:
KM2_forSequence:
TCCGCAATGTGTTATTAAGTTGTCTAASize:
27
Reverse Primer
Name:
KM1_revSequence:
CCGATATGCCTGCCCATCTSize:
19
Probe
Name:
KM-pSequence:
CGTCAATTTGTTTACACCACAATATATCCCGSize:
31
-
Amplicon:
Sequence:
TCCGCAATGTGTTATTAAGTTGTCTAAGCGTCAATTTGTTTACACCACAATATATCCCGAGATGGGCAGGCATATCGGSize:
78