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Detection Image Detection method details

Name:

 QT-TAX-GM-003

Description:

 Quantitative real-time PCR (TaqMan) method for detection of soybean lectin (Le1) gene (EU-RL GMFF GMOMETHODS database as of July 2020).

Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1

Comment:

 Target is the endogenous lectin (Le1) gene from Glycine max which is present as a single copy in the soybean genome.

This assay is recommended for GMO quantitative analysis.

Validation:

 ring trial validation

Standardisation:

 EU reference method

Related Methods:

Type:

 taxon-specific

Target Species name:

 Glycine max

  • Oligonucleotides:

  • Forward Primer

  • Name:

    KVM164

  • Sequence:

    CTTTCTCGCACCAATTGACA

  • Size:

    20

  • Reverse Primer

  • Name:

    KVM165

  • Sequence:

    TCAAACTCAACAGCGACGAC

  • Size:

    20

  • Probe

  • Name:

    TM021

  • Sequence:

    CCACAAACACATGCAGGTTATCTTGG

  • Size:

    26

  • Amplicon:

  • Sequence:

    ctttctcgcaccaattgacannnnnccacaaacacatgcaggttatcttggnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnngtcgtcgctgttgagtttga

  • Size:

    102

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QT-TAX-GM-003Link to the document url. EU reference method protocol 11-08-2020