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Detection Image Detection method details

Name:

 QT-TAX-GM-001

Description:

 Quantitative real-time PCR (TaqMan) method for detection of soybean lectin gene

Comment:

 Target is the endogenous lectin (Le1) gene of Glycine max

Validation:

 ring trial validation

Standardisation:

 ISO standard

Related Methods:

Type:

 taxon-specific

Target Species name:

 Glycine max

  • Oligonucleotides:

  • Forward Primer

  • Name:

    Lectin-F

  • Sequence:

    TCCACCCCCATCCACATTT

  • Size:

    19

  • Reverse Primer

  • Name:

    Lectin-R

  • Sequence:

    GGCATAGAAGGTGAAGTTGAAGGA

  • Size:

    24

  • Probe

  • Name:

    Lectin-TMP

  • Sequence:

    AACCGGTAGCGTTGCCAGCTTCG

  • Size:

    23

  • Amplicon:

  • Sequence:

    tccacccccatccacatttnnnnnnnnnnaaccggtagcgttgccagcttcgnnnnntccttcaacttcaccttctatgcc

  • Size:

    81

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QT-TAX-GM-001Link to the document url. EU reference method protocol
Mbongolo Mbella, E.G., Lievens, A., Barbau-Piednoir, E., Sneyers, M., Leunda-Casi, A., Roosens, N., Van den Bulcke, M. (2011). SYBR Green qPCR methods for detection of endogenous reference genes in commodity crops: a step ahead in combinatory screening of genetically modified crops in food and feed products. Eur Food Res Technol, 232, 485–496.Link to the document url. peer-reviewed article 27-10-2015